Aedes aegypti is the major vector of Dengue in Sri Lanka. Dengue virus is transmitted via blood feeding of vector mosquitoes. Blood is an essential component for the fecundity of mosquitoes and the knowledge on feeding behaviour of Ae. aegypti is important in dengue prevention measures. Therefore, the study was designed to identify the preferential feeding of Ae. aegypti on human ABO blood groups and their impacts on fecundity. Laboratory reared female Ae. aegypti mosquitoes were exposed to all four blood groups at once in separate membrane feeders. After feeding, DNA of blood in mosquitoes was extracted and identified using ABO genotyping PCR. Fecundity was determined by the mean number of eggs in an egg batch. The highest preference was observed for the blood group “O” (P<0.05; 0.00). The mean number of egg production of each blood group was not significantly different showing no impact on the blood type for the fecundity. This study reports for the first time that there is no direct impact of the preferred blood group on the fecundity of Ae. aegypti female mosquitoes. Furthermore, preference of blood group selection by female mosquitoes would be highly important in personal protection measures against mosquito bites, as the personal protection is one of the most effective control measures for prevention of Dengue.
| Published in | American Journal of Entomology (Volume 3, Issue 2) |
| DOI | 10.11648/j.aje.20190302.13 |
| Page(s) | 43-48 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Aedes aegypti, Dengue, ABO Blood Groups, Feeding Behaviour, Fecundity
| [1] | Soumahoro M. K., Fontenille D., Turbelin C., Pelat C., Boyd A., Flahault A. (2010) Imported chikungunya virus infection. Emerging Infectious Diseases 16: 162-163. |
| [2] | Epidemiology unit. 2017. Dengue fever – Sri Lanka disease outbreak news [Internet]. Ministry of Health, Sri Lanka [accessed January 23, 2018]. Available from: http://www.who.int/csr/don/19-july-2017-dengue-sri-lanka/en/ |
| [3] | Scott T. W., Clark G. G., Amerasinghe P. H., Lorenz L. H., Reiter P., Edman J. D. (1993) Detection of multiple blood feeding patterns in Aedes aegypti (Diptera: Culicidae) during a single gonotrophic cycle using a histological technique. Journal of Medical Entomology 30:94-99. |
| [4] | Burkot T. R., Graves P. M., Paru R., Lagog M. (1988) Mixed blood feeding by the malaria vectors in the Anopheles punctuates complex (Diptera: Culicidae). Journal of Medical Entomology 25: 205-213. |
| [5] | Chow E., Wirtz R. A., Scott T. W. (1993) Identification of blood meals in Aedes aegypti by antibody sandwich enzyme-linked immunosorbent assay. Journal of American Mosquito Control Association 9: 196-205. |
| [6] | Ngo K. A., Kramer L. D. (2003) Identification of mosquito blood meals using polymerase chain reaction (PCR) with order specific primers. Journal of Medical Entomology 40: 215-222. |
| [7] | Kent R. J., Norris D. E. (2005) Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. American Journal of Tropical Medicine Hygiene 73: 336-342. |
| [8] | Ponlawat A., Harrington L. C. (2005) Blood feeding patterns of Aedes aegypti and Aedes albopictus in Thailand. Journal of Medical Entomology 42: 844-849. |
| [9] | Siriyasatien P., Pengsakul T., Kittichai V., Phumee A., Kaewsaitiam S., Thavara U., Tawatsin A., Asavadachanukorn P., Mulla M. S. (2010) Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR. Southeast Asian Journal of Tropical Medicine and Public Health 41: 43-47. |
| [10] | Anjomruz M., Oshaghi M. A., Sedaghat M. M., Pourfatollah A. A., Raeisi A., Vatandoost H., Mohtarami F., Yeryan M., Bakhshi H., Nikpoor F. (2014) ABO blood groups of residents and the ABO host choice of malaria vectors in southern Iran. Experimental Parasitology 136: 63-67. |
| [11] | Shirai Y., Funada H., Takizawa H., Seki T., Morohashi M., Kamimura K. (2004) Landing preference of Aedes albopictus (Diptera: Culicidae) on human skin among ABO blood groups, secretors or nonsecretors, and ABH antigens. Journal of Medical Entomology 41: 796-799. |
| [12] | Anand T., Kumar R., Saini V., Meena G. S., Ingle G. K. (2014) Knowledge and use of personal protective measures against mosquito borne diseases in a resettlement colony of Delhi. Annals of Medical and Health Science Research 4: 227-232. |
| [13] | Duong V., Lambrechts L., Paul R. E., Ly S., Lay R. S., Long K. C., Huy R., Tarantola A., Scott T. W., Sakuntabhai A., Buchy P. (2015) Asymptomatic humans transmit dengue virus to mosquitoes. PNAS 112: 14688-14693. |
| [14] | Richards S. L., Lord C. C., Pesko K. A., Tabachnick W. J. (2009) Environmental and biological factors influencing Culex pipiens quinquefasciatus Say (Diptera: Culicidae) vector competence for St. Louis encephalitis virus. American Journal of Tropical Medicine and Hygiene 81: 264-272. |
| [15] | Gonzales K. K., Tsujimoto H., Hansen I. A. (2015) Blood serum and BSA, but neither red blood cells nor haemoglobin can support vitellogenesis and egg production in the dengue vector Aedes aegypti. Peer J 3: e938. |
| [16] | Olayerni C. I., Ande T., Danlami G., Abdullahi U. (2011) Influence of blood meal type on reproductive performance of the malaria vector, Anopheles gambiae ss (Diptera: Culicidae). Journal of Entomology 8: 459-467. |
| [17] | Naksathit A. T., Scott T. W. (1998). Effect of female size on fecundity and survivorship of Aedes aegypti fed only human blood versus human blood plus sugar. Journal of American Mosquito Control Association 14: 148-152. |
| [18] | Lee S. H., Park G., Yang Y. G., Lee S. G., Kim S. W. (2009) Rapid ABO genotyping using whole blood without DNA purification. Korean Journal of. Laboratory Medicine 29: 231-237. |
| [19] | Wood C. S. (1976) ABO blood groups related to selection of human hosts by yellow fever vector. Human Biology 48: 337-341. |
| [20] | Thiemann T. C., Wheeler S. S., Barker C. M., Reisen W. K. (2011). Mosquito host selection varies seasonally with host availability and mosquito density. PLOS Neglected Tropical Diseases 5: e1452. |
| [21] | Jenkins P. V., O’Donnell J. S. (2006) ABO blood group determines plasma von Willebrand factor levels: a biologic function after all?. Transfusion 46: 1836- 1844. |
| [22] | Lenting P. J., Pegon J. N., Christophe O. D., Denis C. V. (2010) Factor VIII and von Willebrand factor–too sweet for their own good. Haemophilia 16: 194-199. |
| [23] | Gallinaro L., Cattini M. G., Sztukowska M., Padrini R., Sartorello F., Pontara E., Bertomoro A., Daidone V., Pagnan A., Casonato A. (2008) A shorter von Willebrand factor survival in O blood group subjects explains how ABO determinants influence plasma von Willebrand factor. Blood 111: 3540-3545. |
| [24] | Day J. F., Edman J. D., Scott T. W. (1994) Reproductive fitness and survivorship of Aedes aegypti (Diptera: Culicidae) maintained on blood, with field observations from Thailand. Journal of Medical Entomology 31: 611-617. |
APA Style
Manushika Prasadini, Dilakshini Dayananda, Sachini Fernando, Iresha Harischandra, Nissanka De Silva. (2019). Blood Feeding Preference of Female Aedes aegypti Mosquitoes for Human Blood Group Types and Its Impact on Their Fecundity: Implications for Vector Control. American Journal of Entomology, 3(2), 43-48. https://doi.org/10.11648/j.aje.20190302.13
ACS Style
Manushika Prasadini; Dilakshini Dayananda; Sachini Fernando; Iresha Harischandra; Nissanka De Silva. Blood Feeding Preference of Female Aedes aegypti Mosquitoes for Human Blood Group Types and Its Impact on Their Fecundity: Implications for Vector Control. Am. J. Entomol. 2019, 3(2), 43-48. doi: 10.11648/j.aje.20190302.13
AMA Style
Manushika Prasadini, Dilakshini Dayananda, Sachini Fernando, Iresha Harischandra, Nissanka De Silva. Blood Feeding Preference of Female Aedes aegypti Mosquitoes for Human Blood Group Types and Its Impact on Their Fecundity: Implications for Vector Control. Am J Entomol. 2019;3(2):43-48. doi: 10.11648/j.aje.20190302.13
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Download@article{10.11648/j.aje.20190302.13,
author = {Manushika Prasadini and Dilakshini Dayananda and Sachini Fernando and Iresha Harischandra and Nissanka De Silva},
title = {Blood Feeding Preference of Female Aedes aegypti Mosquitoes for Human Blood Group Types and Its Impact on Their Fecundity: Implications for Vector Control},
journal = {American Journal of Entomology},
volume = {3},
number = {2},
pages = {43-48},
doi = {10.11648/j.aje.20190302.13},
url = {https://doi.org/10.11648/j.aje.20190302.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aje.20190302.13},
abstract = {Aedes aegypti is the major vector of Dengue in Sri Lanka. Dengue virus is transmitted via blood feeding of vector mosquitoes. Blood is an essential component for the fecundity of mosquitoes and the knowledge on feeding behaviour of Ae. aegypti is important in dengue prevention measures. Therefore, the study was designed to identify the preferential feeding of Ae. aegypti on human ABO blood groups and their impacts on fecundity. Laboratory reared female Ae. aegypti mosquitoes were exposed to all four blood groups at once in separate membrane feeders. After feeding, DNA of blood in mosquitoes was extracted and identified using ABO genotyping PCR. Fecundity was determined by the mean number of eggs in an egg batch. The highest preference was observed for the blood group “O” (PAe. aegypti female mosquitoes. Furthermore, preference of blood group selection by female mosquitoes would be highly important in personal protection measures against mosquito bites, as the personal protection is one of the most effective control measures for prevention of Dengue.},
year = {2019}
}
TY - JOUR T1 - Blood Feeding Preference of Female Aedes aegypti Mosquitoes for Human Blood Group Types and Its Impact on Their Fecundity: Implications for Vector Control AU - Manushika Prasadini AU - Dilakshini Dayananda AU - Sachini Fernando AU - Iresha Harischandra AU - Nissanka De Silva Y1 - 2019/06/26 PY - 2019 N1 - https://doi.org/10.11648/j.aje.20190302.13 DO - 10.11648/j.aje.20190302.13 T2 - American Journal of Entomology JF - American Journal of Entomology JO - American Journal of Entomology SP - 43 EP - 48 PB - Science Publishing Group SN - 2640-0537 UR - https://doi.org/10.11648/j.aje.20190302.13 AB - Aedes aegypti is the major vector of Dengue in Sri Lanka. Dengue virus is transmitted via blood feeding of vector mosquitoes. Blood is an essential component for the fecundity of mosquitoes and the knowledge on feeding behaviour of Ae. aegypti is important in dengue prevention measures. Therefore, the study was designed to identify the preferential feeding of Ae. aegypti on human ABO blood groups and their impacts on fecundity. Laboratory reared female Ae. aegypti mosquitoes were exposed to all four blood groups at once in separate membrane feeders. After feeding, DNA of blood in mosquitoes was extracted and identified using ABO genotyping PCR. Fecundity was determined by the mean number of eggs in an egg batch. The highest preference was observed for the blood group “O” (PAe. aegypti female mosquitoes. Furthermore, preference of blood group selection by female mosquitoes would be highly important in personal protection measures against mosquito bites, as the personal protection is one of the most effective control measures for prevention of Dengue. VL - 3 IS - 2 ER -
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